Regulatory

Part:BBa_J100030

Designed by: Scott Hall   Group: Campbell M Lab   (2011-09-01)


phoA is an inducible promoter induced by phosphate starvation.

phoA should attach to the missing gap in the plasmid to allow transcription. This promoter is induced by phosphate starvation which means that it can limit protein synthesis. The source of phoA is E. Coli and this promoter is suitable for bacterial cells. phoA typically has 76 base pairs. phoA is inactive in its default state with high levels of phosphate. In the absence of phosphate, phoA is activated.

Source: <<http://wolfson.huji.ac.il/expression/vector/Promoters.html#promoters>>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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